Conditions Of Immobilization Stress Research Articles

Peculiarities of changes in a functional condition of pro-oxidant and antioxidant systems in guinea pigs’ lungs in experimental allergic alveolitis under the conditions of immobilization stress

We have analyzed the results of investigation of alterations in indices of pro-oxidant (conjugated diene and malondialdehyde) and antioxidant (superoxide dismutase, ceruloplasmin, catalase) systems in guinea pigs’ lungs in experimental allergic alveolitis in the dynamics of EAA development under the conditions of immobilization stress. The investigation was conducted on 62 female guinea pigs weighing 180-220 g, divided into 4 groups: I – intact guinea pigs (n=20), II – guinea pigs (n=14) with EAA under the conditions of IS (1st day); III – guinea pigs (n=14) with EAA under the conditions of IS (2nd day); IV – guinea pigs (n=14) with EAA under the conditions of IS (34th day). The results of experimental investigation showed that a significant increase in conjugated diene level in animals’ lungs was observed at all stages of EAA development under the conditions of immobilization stress as compared with control group, indicating activation of this marker. The same changes occurred with MDA content, indicating excessive accumulation of this lipid peroxidation product in lung tissue. Intensive synthesis of free radical compounds caused activation of some components of enzymatic system of antioxidant defense. In particular, a moderate decrease in superoxide dismutase activity in lung tissue is observed in response to increased level of LOPs at early stages of EAA and immobilization stress development as compared with the indices in intact animals. The same situation is observed with catalase and ceruloplasmin activity in the lungs of guinea pigs with modeled AA and IS.

Сondition of pro-oxidant and antioxidant systems in guinea pigs’ lungs under the condition of immobilization stress.

We have investigated the results of alterations in indices of pro-oxidant (conjugated diene and malondialdehyde ) and antioxidant (superoxide dismutase, ceruloplasmin, catalase) systems in guinea pigs’ lungs  under the conditions of immobilization stress. The experiment was conducted on 40 female guinea pigs weighing 0.18-0.20 kg. The animals were divided into 4 groups, each contained 10 guinea pigs: I – intact guinea pigs ( control), II–guinea pigs with model of IS on1st day of experiment;Ш–animals on 2nd  day  of experiment;IV- group of animals on 34th day of experimental model of IS.
 The results of our experimental work showed a significant accumulation of lipid peroxidation products in the lung`s tissure in different periods ( on 1st, 2nd and 34th days) of immobilization stress. The state of antioxidant defence was characterized by moderate decrease of inzymes activity (superoxide dismutase, catalase and ceruloplasmin). disorders of balance between pro-oxidant and antioxidant systems couse oxidative stress development.

The State of the Hemostasis System under Conditions of Immobilization Stress against the Background of Experimental Metabolic Syndrome

The State of the Hemostasis System under Conditions of Immobilization Stress against the Background of Experimental Metabolic Syndrome

The State of the Haemostasis System under Conditions of Immobilization Stress against the Background of Experimental Metabolic Syndrome

The State of the Haemostasis System under Conditions of Immobilization Stress against the Background of Experimental Metabolic Syndrome

Peculiarities of changes in a functional condition of pro-oxidant and antioxidant systems in guinea pigs’ lungs in experimental allergic alveolitis under the conditions of immobilization stress

We have analyzed the results of investigation of alterations in indices of pro-oxidant (conjugated diene and malondialdehyde) and antioxidant (superoxide dismutase, ceruloplasmin, catalase) systems in guinea pigs’ lungs in experimental allergic alveolitis in the dynamics of EAA development under the conditions of immobilization stress. The investigation was conducted on 62 female guinea pigs weighing 180-220 g, divided into 4 groups: I – intact guinea pigs (n=20), II – guinea pigs (n=14) with EAA under the conditions of IS (1st day); III – guinea pigs (n=14) with EAA under the conditions of IS (2nd day); IV – guinea pigs (n=14) with EAA under the conditions of IS (64th day). The results of experimental investigation showed that a significant increase in conjugated diene level in animals’ lungs was observed at all stages of EAA development under the conditions of immobilization stress as compared with control group, indicating activation of this marker. The same changes occurred with MDA content, indicating excessive accumulation of this lipid peroxidation product in lung tissue. Intensive synthesis of free radical compounds caused activation of some components of enzymatic system of antioxidant defense. In particular, a moderate decrease in superoxide dismutase activity in lung tissue is observed in response to increased level of LOPs at early stages of EAA and immobilization stress development as compared with the indices in intact animals. The same situation is observed with catalase and ceruloplasmin activity in the lungs of guinea pigs with modeled AA and IS.

Peculiarities of disorders of nitrogen oxide system in the blood at adrenalin-induced myocardial injury in conditions of immobilization stress and their correction by L-arginine

The aim: of the study was to elucidate the changes to nitric oxide activity in the blood during adrenaline-induced myocardial injury under immobilization stress and to establish the corrective effect of L-arginine.
 Methods: determination of free arginine was conducted by the method of Aleinikova T.L., total nitric oxide products in the blood by the method of Schmidt H.H., the total activity of nitric oxide synthase by the method of Sumbaiev V.V.. Immobilization stress was reproduced by the method of Horizontov P.D. Adrenaline-induced myocardial injury was reproduced by the method of Markova O.O. L-arginine was injected based on scientific data by Kiryanova N.A.
 Results. Studies have shown that on days 1 and 3 with adrenaline-induced myocardial injury under immobilization stress there was an increase in nitric oxide products in the blood, respectively, according to control. The use of L-arginine on the 5th day, led to a decrease in levels of NO products in the blood by less than, lower against the group of animals with MI and IS, to treatment.
 Conclusions. Thus, biochemical studies of NO system in the dynamics of IS and MI showed an increase in food content and total synthase activity of NO on the background of reduced levels of L-arginine, which were detected at all stages of the study and especially expressed on the 1st day before treatment. The use of the drug L-arginine, made it possible to identify its corrective effect on impaired metabolic processes in MI and IS

Condition of pro-oxidant and antioxidant systems in guinea pigs’ lungs under the condition of immobilization stress

We have investigated the results of alterations in indices of pro-oxidant (conjugated diene and malondialdehyde) and antioxidant (superoxide dismutase, ceruloplasmin, catalase) systems in guinea pigs’ lungs under the conditions of immobilization stress. The experiment was conducted on 40 female guinea pigs weighing 0.18-0.20 kg. The animals were divided into 4 groups, each contained 10 guinea pigs: I – intact guinea pigs (control), II–guinea pigs with model of IS on1st day of experiment;Ш–animals on 2nd  day of experiment;IV- group of animals on 34th day of experimental model of IS.
 The results of our experimental work showed a significant accumulation of lipid peroxidation products in the lung`s tissure in different periods (on 1st, 2nd and 34th days) of immobilization stress. The state of antioxidant defence was characterized by moderate decrease of inzymes activity (superoxide dismutase, catalase and ceruloplasmin). disorders of balance between pro-oxidant and antioxidant systems couse oxidative stress development.

ФУНКЦІОНАЛЬНИЙ СТАН ПРООКСИДАНТНОЇ ТА АНТИОКСИДАНТНОЇ СИСТЕМ У ЛЕГЕНЯХ ЗА УМОВ ЕКСПЕРИМЕНТАЛЬНОГО АЛЬВЕОЛІТУ І СТРЕСУ

We have analysed the results of the study of alterations in indices of pro-oxidant (conjugated diene and malondialdehyde) and antioxidant (superoxide dismutase, ceruloplasmin, catalase) systems in guinea pigs’lungs in experimental allergic alveolitis in the dynamics of EAA development under the conditions of immobilization stress. The study included 62 female guinea pigs weighing 180-220 g, divided into 4 groups: 1 – intact guinea pigs (n=20); 2 – guinea pigs (n=14) with EAA under conditions of IS (1st day); 3 – guinea pigs (n=14) with EAA under conditions of IS (2nd day); 4 – guinea pigs (n=14) with EAA under conditions of IS (34th day). The results of the experimental study showed that a signifi cant increase in conjugated diene level in animal lungs was observed at all stages of EAA development under conditions of immobilization stress as compared to a control group, indicating the activation of this marker. The same changes occurred with MDA content, indicating excessive accumulation of this lipid peroxidation product in lung tissue. Intensive synthesis of free radical compounds caused activation of some components of the enzymatic system of antioxidant defence. In particular, a moderate decrease in superoxide dismutase activity in lung tissue is observed in response to an increased level of LOPs at early stages of EAA and immobilization stress development as compared to these indices in intact animals. The same situation is observed with catalase and ceruloplasmin activity in the lungs of guinea pigs with modelled AA and IS.

The Role of Alpha-Tocopherol and Cycloferon in the Regulation of Apoptosis in Neurons of the Hypothalamic Suprachiasmatic Nucleus and Pinealocytes during Stress and Aging

The suprachiasmatic nucleus (SCN) of the hypothalamus and the pineal gland (epiphysis) play an important role not only in the regulation of circadian rhythms but also in the implementation of adaptive responses, including those to various stress factors. Age-related morphofunctional changes in these brain structures, including those associated with increased oxidative stress, exert a significant effect on the organism as a whole. The aim of this work was to explore the dynamics and mechanism of apoptosis in pinealocytes and SCN neurosecretory cells as well as to determine the possibilities of pharmacological correcting this process by an antioxidant alpha-tocopherol and an immunomodulator cycloferon under physiological and immobilization stress conditions in young (2–4-month-old) and aged (30-month-old) Wistar rats. The preparations were administered perorally once a day for 14 days. While the apoptosis level increased with age both in the SCN and the pineal gland, administration of alpha-tocopherol, cycloferon and their combination led to abate this process. It was shown that stress-induced apoptosis in the SCN and the pineal gland proceeds via the p53-dependent pathway, while administration of alpha-tocopherol acetate, cycloferon and their combination decreases the apoptosis level in pinealocytes, suppressing p53 expression both in young and aged animals. In the SCN, no relationship was found between apoptosis and p53 expression levels after administration of the above preparations during stress, suggesting the involvement of different mechanisms.

СТАН СИСТЕМИ ПРОТЕЇНАЗИ/ІНГІБІТОРИ ПРОТЕЇНАЗ У ЩУРІВ У ДИНАМІЦІ ІММОБІЛІЗАЦІЙНОГО СТРЕСУ НА ТЛІ ГІПОТИРЕОЗУ

Вступ. У патогенезі багатьох патологічних станів важливе місце посідають порушення протеїназо-інгібіторної системи. При відсутності належного контролю за протеолізом розвиваються патологічні стани, що супроводжуються виникненням деструктивних, запальних та імунних реакцій. Ряд дослідників встановив захисну дію йодовмісних тиреоїдних гормонів при стресі, які реалізуються в результаті їх взаємодії з клітинним геномом, що призводить до стимуляції локальних стрес-лімітуючих систем.
 Мета дослідження – встановити особливості функціонування системи протеїнази/інгібітори протеїназ за умов іммобілізаційного стресу на тлі зниження рівня йодовмісних гормонів щитоподібної залози.
 Методи дослідження. Гіпотиреоз моделювали, щоденно вводячи тваринам per os тиреостатик мерказоліл (“Здоров’я”, Україна) у дозі 25 мг/кг протягом 21-ї доби. Гострий іммобілізаційний стрес моделювали шляхом прив’язування піддослідних щурів у положенні на спині за 4 кінцівки без обмеження рухо­мості голови тривалістю 3 год.Для дослідження концентрації йодовмісних гормонів щитоподібної залози, активності протеїназної системи і вмісту інгібіторів протеолізу використано спектрофотометричні та імуноферментні методи.
 Результати й обговорення. Досліджено вплив іммобілізаційного стресу в щурів з попередньо змодельованим гіпотиреозом на показники протеїназо-інгібіторної системи крові. На стадії тривоги розвитку стрес-реакції в евтиреоїдних тварин встановлено підвищення протеолітичної активності крові на тлі збільшення вмісту α1-інгібітора протеаз та α2-макроглобуліну, що вказує на посилення антипротеолітичного потенціалу для стримування розвитку деструкції.На стадії резистентності відбувалася стабілізація протеолітичної активності крові. Однак при тривалому стресі (стадія виснаження) вона знову достовірно зростала на тлі зниження активності інгібіторів протеаз, що свідчить про виснаження захисного інгібіторного резерву. У тварин з гіпотиреозом на всіх стадіях розвитку стрес-реакції мало місце достовірне підвищення протеолітичної активності крові, причому показники інгібіторів протеаз достовірно зменшувалися. Це призводило до різкого зростання індексу протеолізу, що відбувалося на тлі збільшення проникності лізосомальних мембран.
 Висновок. На тлі гіпотиреозу спостерігають інтенсивніше, ніж у евтиреоїдних тварин, зростання протеолітичної активності крові на фоні пригнічення антипротеазної активності та збільшення проникності лізосомальних мембран.

Influence of extract of peach ordinary (persica vulgaris) leaves on the state of thymic-lymphatic element of the immune system of rats in conditions of chronic immobilization stress

The aim – study of the influence of thick extract of peach ordinary (Persica vulgaris) leaves (TEPL) on the state of thymic-lymphatic element of the immune system of rats in conditions of chronic immobilization stress. Materials and methods. The model of chronic immobilization stress was reproduced for 18 days by daily four hour immobilization of rats in tight box. Investigated TEPL, which was obtained at the Department of Chemistry of Natural Compounds of National University of Pharmacy from peach leaves of the Salve variety harvested in Tajikistan was administered intragastrically in a conditionally effective dose of 100 mg/kg. Reference drug the syrup «Immuno-Ton» was used in a dose of 3 ml/kg. The state of thymic-lymphatic element of the immune system in conditions of chronic immobilization stress was determined after the euthanasia of animals under mild inhalation anesthesia by the results of the study of the coefficients of thymus and spleen mass and histological studies on micropreparations, which were prepared according to the generally accepted method. To assess the nature of the effect of the TEPL on the condition of the organs of the rat thymic-lymphatic system under chronic immobilization stress conditions, a comparison was made with intact control and control pathology. Results. It was determined that chronic immobilization stress had suppressive effect on the thymic-lymphatic element of the immune system. An increase in the width of the marginal zone of lymphoid follicles and muft and the number of perivascular lymphatic clusters 1.2 times (p <0.05) compared with control pathology, reduction of signs of stressful hypoxia - the number of structures of white pulp with spasm of the central arteries was noted in the spleen. Reducing the degree of change from the third to fourth phase to the first phase of the accident transformation is set in the thymus, which is confirmed by an increase in the mass of the thymus mass by 2.7% in comparison with the control pathology. Conclusions. The ability of TEPL to restore impaired activity of organs of thymic-lymphatic element of the immune system has been proved. It is likely, that polyphenolic compounds of TEPL exhibit antioxidant properties, enhancing the activity of the antioxidant system, and polysaccharides exhibit immunostimulative properties, the result of which is a stressprotective action. The effectiveness of TEPL was not inferior to the reference drug «Immuno-Ton»

Oxidative Stress as a Mechanisms of Reduced Glucose Absorption under Conditions of Immobilization Stress.

Experimental studies demonstrated inhibition of glucose absorption in the jejunum in acute and chronic 1-h daily immobilization stress, with the maximum inhibition on day 7 of immobilization. These changes correlate with the development of oxidative stress in animals over the entire duration of the experiment, which manifested by an increase in the content of the primary and end LPO products and decrease of the total antioxidant activity of the blood. Correction of these shifts with the antioxidant drug inhibits accumulation of LPO products and increases antioxidant defense and glucose absorption rate. These data prove the important role of peroxidation in regulation of glucose absorption.

Peculiarities of changes in a functional condition of pro-oxidant and antioxidant systems in guinea pigs' lungs in experimental allergic alveolitis under the conditions of immobilization stress

Peculiarities of changes in a functional condition of pro-oxidant and antioxidant systems in guinea pigs' lungs in experimental allergic alveolitis under the conditions of immobilization stress

Chronic Psychological Stress Accelerates Vascular Senescence and Impairs Ischemia-Induced Neovascularization: The Role of Dipeptidyl Peptidase-4/Glucagon-Like Peptide-1-Adiponectin Axis.

BackgroundExposure to psychosocial stress is a risk factor for cardiovascular disease, including vascular aging and regeneration. Given that dipeptidyl peptidase‐4 (DPP4) regulates several intracellular signaling pathways associated with the glucagon‐like peptide‐1 (GLP‐1) metabolism, we investigated the role of DPP4/GLP‐1 axis in vascular senescence and ischemia‐induced neovascularization in mice under chronic stress, with a special focus on adiponectin ‐mediated peroxisome proliferator activated receptor‐γ/its co‐activator 1α (PGC‐1α) activation.Methods and ResultsSeven‐week‐old mice subjected to restraint stress for 4 weeks underwent ischemic surgery and were kept under immobilization stress conditions. Mice that underwent ischemic surgery alone served as controls. We demonstrated that stress impaired the recovery of the ischemic/normal blood‐flow ratio throughout the follow‐up period and capillary formation. On postoperative day 4, stressed mice showed the following: increased levels of plasma and ischemic muscle DPP4 and decreased levels of GLP‐1 and adiponectin in plasma and phospho‐AMP‐activated protein kinase α (p‐AMPKα), vascular endothelial growth factor, peroxisome proliferator activated receptor‐γ, PGC‐1α, and Sirt1 proteins and insulin receptor 1 and glucose transporter 4 genes in the ischemic tissues, vessels, and/or adipose tissues and numbers of circulating endothelial CD31+/c‐Kit+ progenitor cells. Chronic stress accelerated aortic senescence and impaired aortic endothelial sprouting. DPP4 inhibition and GLP‐1 receptor activation improved these changes; these benefits were abrogated by adiponectin blocking and genetic depletion.ConclusionsThese results indicate that the DPP4/GLP‐1‐adiponectin axis is a novel therapeutic target for the treatment of vascular aging and cardiovascular disease under chronic stress conditions.

Effects of Hint1 deficiency on emotional-like behaviors in mice under chronic immobilization stress.

BackgroundHistidine triad nucleotide‐binding protein 1 (HINT1) is regarded as a haplo‐insufficient tumor suppressor and is closely associated with diverse neuropsychiatric diseases. Moreover, HINT1 is related to gender‐specific acute behavior changes in schizophrenia and in response to nicotine. Stress has a range of molecular effects in emotional disorders, which can cause a reduction in brain‐derived neurotrophic factor (BDNF) expression in the hippocampus, resulting in hippocampal atrophy and neuronal cell loss.MethodsThis study examined the role of HINT1 deficiency in anxiety‐related and depression‐like behaviors and BDNF expression in the hippocampus under chronic immobilization stress, and investigated whether the sex‐specific and haplo‐insufficient effects exist in emotional‐like behaviors under the same condition.ResultsIn a battery of behavior tests, the results of the control group, not exposed to stress, showed that knockout (KO) and heterozygosity (HT) of Hint1 had anxiolytic‐like and antidepression‐like effects on the male and female mice. However, both male and female Hint1‐KO mice showed elevated anxiety‐related and antidepression‐like behavior under chronic immobilization stress; moreover, both male and female Hint1‐HT mice displayed elevated anxiety‐related behavior and increased depression‐like behavior under chronic immobilization stress. There were no significant differences in general locomotor activity between Hint1‐KO and ‐HT mice and their wild‐type (WT) littermates. Hint1‐KO mice under basal and chronic immobilization stress conditions expressed more BDNF in the hippocampus than did Hint1‐HT and WT mice; overall, there were no significant sex differences in emotional‐like behaviors of Hint1‐KO and ‐HT mice. Additionally, Hint1‐HT mice showed haplo‐insufficient effects on emotional‐like behaviors under basic conditions, rather than under chronic immobilization stress.ConclusionsBoth male and female HINT 1 KO and HT mice had a trend of anxiolytic‐like behavior and antidepression‐like behavior at control group. However, both male and female HINT1 KO mice showed elevated anxiety‐related and antidepression‐like behavior under chronic immobilization stress; moreover, both male and female HINT1 HT mice displayed elevated anxiety‐related behavior and increased depression‐like behavior under chronic immobilization stress.

Role of NF-κB, PI3K, MAPK/ERK 1/2, and p38 in Erythropoietin Production by Bone Marrow Nuclears under Conditions of Immobilization Stress.

The involvement of the studied signal cascades in the regulation of erythropoietin production by bone marrow nuclears under conditions of immobilization stress depends on the type of the hemopoiesis-inducing microenvironment cells and the period of blood system reaction to stress exposure. Secretory activity of monocytes is regulated mainly by PI3K improving cell resistance to disturbances. The functional role of signal cascades involved in the production of erythropoietin by T cells is determined by the stage of the common adaptation syndrome.

Biochemical parameters and adaptation of white rats with active and passive type of behavior under immobilization stress.

The aim was to study dynamics of changes of certain biochemical indices and the development of adaptation in white rats with active and passive type of behavior under the influence of immobilization stress. Experiments were conducted on white rats, previously divided into groups of active and passive ones, on the basis of indicators of activity in the test “open field”. Modeling of immobilization stress was carried out by placing the animals in plastic boxes for 6 hours daily within 30 days. It was found that the immobilization caused changes of carbohydrate, lipid, calcium metabolism, activity of alkaline phosphatase, alanine- and aspartataminotransferase in blood serum of both experimental groups. However, the intensity, direction of the biochemical parameters, development in time depends on the type of animal behavior. To a greater degree active animals reacted to immobilization by changing of concentration of glucose and calcium in the blood on the 3rd and 5th day of the experiment, changes of all investigated parameters were observed on the 10th and 30th day of the experiment. Animals with active type of behavior appeared to be more susceptible to stress mobilization, but the intensity of their metabolic processes was higher than in animals with a passive type of behavior. This is testified by the highest level of blood glucose and lower levels of total cholesterol and triglycerides, as well as more active processes of calcium metabolism in the active animals than passive ones. Thus, dynamics of changes of biochemical parameters and the proceeding of the adaptation process in the conditions of immobilization stress depends on individually-typological features of animal behavior. This indicates to the necessity of differentiated approach to correction of adaptation disorders due to the action of immobilization stress on the organism with different typology of behavior.

ОСОБЛИВОСТІ АКТИВНоСТІ ТРАНСАМІНАЗ У КРОВІ ТА ПЕЧІНЦІ ПРИ ЕКСПЕРИМЕНТАЛЬНІЙ ПНЕВМОНІЇ В УМОВАХ ІММОБІЛІЗАЦІЙНОГО СТРЕСУ ТА ВПЛИВ НА НИХ КОРВІТИНУ

&lt;p&gt;РЕЗЮМЕ. Експериментальна пневмонія в умовах іммобілізаційного стресу супроводжується значним&lt;br /&gt;зростанням показників АЛТ та АСТ в крові і печінці, а застосування корвітину привело до зниження активності&lt;br /&gt;трансаміназ, що свідчить про його позитивний корегувальний вплив на ці показники.&lt;/p&gt;&lt;p&gt;&lt;br /&gt;КЛЮЧОВІ СЛОВА: експериментальна пневмонія, стрес, аланінамінотрансфераза, аспартатамінотрансфераза, корвітин.&lt;/p&gt;

[The ultrastructure of Leydig cells under the influence of drinking mineral water and electromagnetic radiation under the stress conditions in the rats].

The objective of the present study was elucidate the peculiar features of low-intensity electromagnetic radiation (LI EMR) and mineral water (MW) on the ultrastructure of rat Leydig cells under conditions of immobilization stress. The experiments were carried out on outbred male rats with the use of electron microscopy. It has been demonstrated that the prophylactic consumption of drinking sulfate-containing mineral water and the application low-intensity electromagnetic radiation (with the flow power density of 1 mcW/cm2 and frequency around 1,000 Hz) or the combination of these two modalities under conditions of immobilization stress reduced the degree of ultrastructural derangement in the rat Leydig cells and stimulated the development of regenerative processes. In the cases of the single-factor impact, drinking mineral water exerted more pronounced action than low-intensity electromagnetic radiation on mitochondrial regeneration. In case of the simultaneous application of the two factors their protective action on the Leydig cells was much more conspicuous than that of either of them applied alone. It is concluded that drinking sulfate-containing mineral water in combination with the application of low-intensity electromagnetic radiation enhances resistance of the rat Leydig cells to stress.

[The combined action of drinking mineral water and low-intensity electromagnetic radiation under the immobilization stress conditions (an experimental study)].

The present study carried out on white male rats in experiments with the use of biochemical, radioimmunological, and electron- microscopic methods. It was shown that the combined treatment with potable mineral water (MV) and low-intensity electromagnetic radiation (LIEMR) of ultrahigh frequency (power density less than 1 pW/cm2, the frequency about 1000 MHz) facilitated the activation of metabolic and intracellular regenerative processes in the liver and testes. One of the advantages of the combined application of MV and LIEMR over the single-factor treatment manifested itself as the weakening of stress reactions, the increase in the frequency of the plastic processes, and the more harmonious development of different forms of intracellular regeneration. The results of the study provide a deeper insight ino the mechanisms underlying the combined actions of drinking mineral water and low-intensity electromagnetic radiation; also, they justify the application of these factors for the protection of the reproductive system and the entire body from stress-induced disorders.